Important processes of this stage include cell expansion, differentiation, and accumulation of reserve substances essential for germination and regeneration of somatic embryos (Mishra et al. Maturation is a crucial step for the success of somatic embryogenesis. 1991), represents a valuable tool for developing cultures of economic importance, since it has great potential for the mass propagation of elite plants (Gupta et al. The use of somatic embryogenesis, an in vitro culture technique in which single cells or small groups of somatic cells give rise to embryos (Tautorus et al.

The endogenous carbohydrate profile may be a valuable parameter for developing optimized protocols for the maturation of somatic embryos in papaya. Concomitant with the increase in the differentiation of somatic embryos in PEG cultures, there was an increase in the endogenous content of sucrose and starch, which appears to be related to a rising demand for energy, a key point in the conversion of C. Histomorphological analysis of embryonic cultures with PEG revealed meristematic centers containing small isodiametric cells with dense cytoplasm and evident nuclei. In both control and PEG treatments, pro-embryogenic differentiation to the cotyledonary stage was observed and was significantly higher with PEG treatment. PEG treatment (6%) increased the number of normal mature somatic embryos followed by somatic plantlet production. papaya, we analyzed somatic embryo development and carbohydrate profile changes during maturation treatments with PEG (6%) or without PEG (control). To study the effects of PEG on somatic embryogenesis of C. Addition of polyethylene glycol (PEG), a nonplasmolyzing osmotic agent, to a maturation medium increases the production of somatic embryos in C. Efficient protocols for somatic embryogenesis of papaya ( Carica papaya L.) have great potential for selecting elite hybrid genotypes.

Carbs in ze a rita